Purification of recombinant proteins pdf
ثبت نشده
چکیده
منابع مشابه
Design and Construction of Recombinant ELP-Intein Cassette for Use in Simple and new Purification Methods of Recombinant Proteins
Background and Objective: Use of elastin-like proteins (ELPs) provides high-performance protein purification without need for chromatography. In line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from ELP b...
متن کاملEvaluation and comparison of affinity chromatography and precipitation– based methods on purification of recombinant streptokinase
Background: Increase of protein purity is a serious challenge in the production of recombinant therapeutic proteins. For this purpose, several strategies have been employed to purify the target protein, among which the affinity chromatography-based purification methods and tagged proteins such as Ni-NTA are common and but costly. Therefore column-free purification techniques, such as using elas...
متن کاملAffinity Based Nano-Magnetic Particles for Purification of Recombinant Proteins in Form of Inclusion Body
Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...
متن کاملPurification of Large Quantities of Biologically Active Recombinant Human Growth Hormone
Production and purification of human growth hormone using a simple method was studied in two recombinantEscherichia coli, D7-5 and C27-2 strains. The r-hGH was expressed in the form of inclusion body in a batchfermentation process and purified to 99% purity using a procedure based on acid precipitation of the hostderived proteins and other impurities. The effect of the pH and ...
متن کاملPersian sturgeon growth hormone elaboration and purification
In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2015